MINIDIGI 1A DRIVER DETAILS:
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MINIDIGI 1A DRIVER
Supplied with two CV-7B headstages enabling the unit to perform as minidigi 1a patch clamps, two current clamps or a combination of patch and current amplifiers. This is a high compliance voltage clamp and current clamp amplifier.
This option requires no OS understanding. The oocyte was continuously superfused minidigi 1a a gravity-feed system that allowed up to eight different solutions to be independently applied. The optical unit comprised a fluorescence objective mounted on a stable enclosure that housed the filter set and associated components. The optical unit was mounted on an X-Y translation stage M, Newport that allowed centering of the oocyte within the excitation beam.
Fluorescence was excited by a W halogen light source powered from a stabilized DC source. For stability the light was operated continuously during the minidigi 1a session and an electronic shutter VST1, Uniblitz, Vincent Assoc.
The diode and lens were mounted in a minidigi 1a enclosure at the side of the optical unit. This allowed independent subtraction of the background fluorescence signal from the photodiode output using a stable, low noise, adjustable voltage reference before final amplification and filtering.
Changes in fluorescence were measured in response to changing substrate concentrations and changing membrane potential. For the normalized data in the voltage jump experiments, F is expressed in arbitrary units minidigi 1a.
Each test substrate concentration was bracketed with a control solution to allow for correction of fluorescence rundown. The fluorescence signal was acquired after the new superfusate had been applied minidigi 1a 1. After equilibration, the shutter was opened 7—10 times in succession for ms at 2.
Dasylab 7 driver for minidigi 1a axon instruments - NI Community - National Instruments
The data were minidigi 1a for fluorescence rundown see below. Steady-state fluorescence measurements. After we equilibrated the oocyte with a new test solution, the shutter was opened periodically for seven successive ms pulses followed by a 2-s closing time. minidigi 1a
To control for fluorescence rundown, each test solution was bracketed by ND solution. For better visualization of the rundown, the ND data is connected by a dotted line drawn by eye. These step changes in membrane potential are the cause of the capacitive current spikes visible in the current recording. When the shutter is minidigi 1a, F is outside the measurable range.
Continuous lines are fits to data using the Hill equation. Data were fitted with the Hill equation with H constrained to 1. Data for individual oocytes were fit with the Hill equation Eq. See text for all minidigi 1a parameters.
Axon Instruments Minidigi 1A Two-Channel Acquisition System
This also allowed us to follow the time course of the voltage-induced fluorescence change by matching the bandwidths of the current and fluorescent signals. The amount of offset minidigi 1a was estimated from the data acquired using the steady-state experiments described above. For presteady-state fluorescence measurements, the voltage jump protocol was modified by shortening the time spent at each target potential to 40 ms to reduce photobleaching and by increasing the number of averages to 64 to improve the signal-to-noise ratio minidigi 1a sacrificing signal bandwidth, which was set to Hz for both the current and F signals. During the course of an experiment, fluorescence decreased, and this decrease fell into two categories. For the steady-state experiments, we plotted the fluorescence data acquired in the control solution as a function of time elapsed after the start of the experiment in these experiments the contribution of photobleaching to the fluorescence decrease was minimal.
minidigi 1a We fitted the data with a single decaying exponential plus an offset, which takes into account that the fluorescence will never reach zero even a nonlabeled oocyte gives a definite fluorescence signal when placed in the experimental chamber. In both cases, the approach was validated, because it successfully corrected the fluorescence change seen in control solutions during the course of an experiment. The fluorescence response to a change in substrate minidigi 1a, determined for different membrane voltages, was fitted with the modified Hill equation of the form.
MiniDigi 1A Properties
See all. Item Information Condition:. Sign in to check out Check out as guest. Make Offer. Resume making your offerif the page does not update immediately. Add to watchlist Remove from watch list. Watch list is full. Longtime Member.The MiniDigi 1A is a small hardware device that is shipped with pCLAMP with orders of pCLAMP 10 except orders that are upgrades from. Axon™ MiniDigi™ Setup Guide Download Page. Published 01/21/ PM Updated 02/14/ AM. Download a PDF version of minidigi 1a Axon MiniDigi.